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lambda kappa fitc  (SouthernBiotech)


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    Structured Review

    SouthernBiotech lambda kappa fitc
    Lambda Kappa Fitc, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+lambda+fitc/Goat+Anti-Human+Kappa-FITC/pmc12551279-59-73-80
    Average 93 stars, based on 52 article reviews
    lambda kappa fitc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Crimean-Congo Hemorrhagic Fever Survivors Elicit Protective Non-Neutralizing Antibodies that Target 11 Overlapping Regions on Viral Glycoprotein GP38
    Article Snippet: Briefly, soluble cytosolic protein (SCP) and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). .. Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA) and incubated on ice for 20 min. Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech, dilution 1:100), anti-human lambda FITC (Southern Biotech, dilution 1:100), and PI (Invitrogen, dilution 1:100) for 20 minutes on ice. .. Yeast were again washed two times and samples were analyzed on a BD FACSCanto II flow cytometer (BD Biosciences).

    Article Title: Reprogramming the antigen specificity of B cells using genomeediting technologies
    Article Snippet: .. MGRM8 or WITO probes were incubated with either MGRM8 or WITO APC labeled tetramer as previously described at their EC10 concentrations along with 1000x dilution of anti-human lambda FITC-labeled antibody (Southern Biotech) for 45min. ..

    Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38
    Article Snippet: Briefly, soluble cytosolic protein (SCP) and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). .. Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA) and incubated on ice for 20 min. Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech, dilution 1:100), anti-human lambda FITC (Southern Biotech, dilution 1:100), and PI (Invitrogen, dilution 1:100) for 20 min on ice. .. Yeast were again washed two times and samples were analyzed on a BD FACSCanto II flow cytometer (BD Biosciences).

    Cellenone:

    Article Title: Crimean-Congo Hemorrhagic Fever Survivors Elicit Protective Non-Neutralizing Antibodies that Target 11 Overlapping Regions on Viral Glycoprotein GP38
    Article Snippet: Briefly, soluble cytosolic protein (SCP) and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). .. Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA) and incubated on ice for 20 min. Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech, dilution 1:100), anti-human lambda FITC (Southern Biotech, dilution 1:100), and PI (Invitrogen, dilution 1:100) for 20 minutes on ice. .. Yeast were again washed two times and samples were analyzed on a BD FACSCanto II flow cytometer (BD Biosciences).

    Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38
    Article Snippet: Briefly, soluble cytosolic protein (SCP) and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). .. Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA) and incubated on ice for 20 min. Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech, dilution 1:100), anti-human lambda FITC (Southern Biotech, dilution 1:100), and PI (Invitrogen, dilution 1:100) for 20 min on ice. .. Yeast were again washed two times and samples were analyzed on a BD FACSCanto II flow cytometer (BD Biosciences).

    Staining:

    Article Title: Crimean-Congo Hemorrhagic Fever Survivors Elicit Protective Non-Neutralizing Antibodies that Target 11 Overlapping Regions on Viral Glycoprotein GP38
    Article Snippet: Briefly, soluble cytosolic protein (SCP) and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). .. Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA) and incubated on ice for 20 min. Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech, dilution 1:100), anti-human lambda FITC (Southern Biotech, dilution 1:100), and PI (Invitrogen, dilution 1:100) for 20 minutes on ice. .. Yeast were again washed two times and samples were analyzed on a BD FACSCanto II flow cytometer (BD Biosciences).

    Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38
    Article Snippet: Briefly, soluble cytosolic protein (SCP) and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). .. Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA) and incubated on ice for 20 min. Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech, dilution 1:100), anti-human lambda FITC (Southern Biotech, dilution 1:100), and PI (Invitrogen, dilution 1:100) for 20 min on ice. .. Yeast were again washed two times and samples were analyzed on a BD FACSCanto II flow cytometer (BD Biosciences).

    Labeling:

    Article Title: Reprogramming the antigen specificity of B cells using genomeediting technologies
    Article Snippet: .. MGRM8 or WITO probes were incubated with either MGRM8 or WITO APC labeled tetramer as previously described at their EC10 concentrations along with 1000x dilution of anti-human lambda FITC-labeled antibody (Southern Biotech) for 45min. ..

    other:

    Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38.
    Article Snippet: Yeast were again washed two times and samples were analyzed on a BD FACSCanto II flow cytometer (BD Biosciences).



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38

    doi: 10.1016/j.celrep.2024.114502

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Samples were incubated for 30 min on ice with a cocktail of streptavidin Alexa Fluor 633 (Invitrogen; to detect bound GP38), goat F(ab')2 anti-human kappa FITC and goat F(ab')2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen; to detect cell viability).

    Techniques: Derivative Assay, Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Membrane, Cell Isolation, Mutagenesis, Expressing, Plasmid Preparation, Software, Affinity Column, Sequencing, Transfection, Electron Microscopy

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38

    doi: 10.1016/j.celrep.2024.114502

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA) and incubated on ice for 20 min. Yeast cells were then washed two times in PBSF and further stained with a cocktail of ExtraAvidin-R-PE (Sigma Aldrich, dilution 1:50), anti-human kappa FITC (Southern Biotech, dilution 1:100), anti-human lambda FITC (Southern Biotech, dilution 1:100), and PI (Invitrogen, dilution 1:100) for 20 min on ice.

    Techniques: Derivative Assay, Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Membrane, Cell Isolation, Mutagenesis, Expressing, Plasmid Preparation, Software, Affinity Column, Sequencing, Transfection, Electron Microscopy